Metagenomes of Pisum sativum phyllosphere - SRA

SRX24360983: Metagenomes of Pisum sativum phyllosphere
1 ILLUMINA (NextSeq 500) run: 996,056 spots, 298.8M bases, 108.5Mb downloads

Design: Whole leaves were collected in field and stored in sterile 10mM MgCl2 for transport to lab for processing. Leaves were sonicated in fresh buffer for 10 minutes then vortex to dislodge epiphytic bacteria into solution. Leaf wash was passed through a 5um filter to remove plant cells prior to DNA extraction with Qiagen DNAeasy Blood and Tissue kit. Extraction was performed to kit standards with an additional bead beating with 0.4mm silicon microbears at 4 M/S for 15 seconds following the addition of lysis buffer. Metagenomic sequencing was performed at the Cornell Biotechnology Resource Center with Nextera WGS library preparation and Illumina NextSeq500 paired-end 2 x 150bp reads.

Submitted by: Cornell University

Study: Pseudomonads in the Phyllosphere

PRJNA961062 • SRP503869 • All experiments • All runs

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Pseudomonads in the Phyllosphere. The goal of this project is to examine the genetic diversity of fluorescent isolates, with a particular interest in Pseudomonads, inhabiting the Pisum phyllosphere.

Sample: Leaf w/o Aphids

SAMN41075073 • SRS21118447 • All experiments • All runs

Organism: metagenome

Library:

Name: F9_WO_3

Instrument: NextSeq 500

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 996,056 spots, 298.8M bases, 108.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR28796952	996,056	298.8M	108.5Mb	2025-01-08

ID:: 32675639

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